Flow Cytometry Stem Cell Enumeration

Multicolor flow cytometry stem cell research t cell research support support sales and support faq technical support training resources and tools.

Flow cytometry stem cell enumeration.

Several flow cytometric studies have shown. In the present study we review and compare the major counting techniques of stem and progenitor cells. The advent and use of a cd34 monoclonal antibody enabled the detection of these cells by flow cytometry. Issues in enumeration applications of flow cytometry in stem cell research and tissue regeneration 10 1002 9780470631119 115 134 2012.

Flow cytometric enumeration of cd34 hematopoietic stem and progenitor cells hscs is widely used for evaluation of graft adequacy of peripheral blood and bone marrow stem cell grafts. Identification of cd34 hematopoietic stem cells cd34 cell enumeration by flow cytometry is a rare event analysis. Centers for disease control. I am a development technologist for the human cellular therapy laboratory at mayo clinic.

Enumeration of cd34 cells in cord blood. Robert sutherland hematopoietic stem cells. Hematopoietic progenitor cells or hpcs are used to reconstitute hematopoiesis following marrow ablative therapies. Bd stem cell enumeration starter kit includes software for first time users.

For the analysis of cd34 hscs cell samples are stained with fluorescently labeled antibodies against both the hsc antigen cd34 and the pan leukocyte antigen cd45. Some of the cytometry tests we offer. Cytokine and chemokine profiling. Size 50 tests status ivd cat no 656296.

Furthermore cd34 cell enumeration is useful for monitoring the stem cell yield during graft manipulations such as immunomagnetic selections ex vivo expansion or genetic modifications of hscs. The cd34 antigen is stage specific and identifies cells in the early stages of hematopoietic differentiation. A variation on a single platform flow cytometric method based on the ishage gating strategy. Detection of minimal residual disease.